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Transportation Data and Analytics / Highway Data / Intercity Highway Mileage
FDOT provides official highway mileage between cities in Florida. An online calculator, an Excel spreadsheet and a location map are available. The spreadsheet includes the city-to-city mileage matrix itself, a list of centroids (intersections within each city that define the city location), and an information page. The map shows the location of each city included in the Official Highway Mileage Viewer.
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Florida Department of Transportation